Role of thyroid hormones, TGF-beta1 and ON in arterial hypertension generated by environmental toxins

ROMERO CAÍMI, GISELLE; GUTIERREZ, CHRISTOPHER P. A.; DEZA, SAHIRA; BONAZZOLA, PATRICIA; CHIAPPINI, FLORENCIA; CASTILLA, ROCÍO; ALVAREZ, LAURA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

la Sociedad Argentina de Investigación Clínica

Hypertension is one of the main pathologies associated with environmental pollution. Among the environmental pollutants is hexachlorobenzene (HCB). We demonstrate that HCB increases blood pressure (BP) and alters morphology and vascular functionality (VF) in vivo. TGF-β1 and RE-α are involved in these effects. Since the effects on the VF were endothelium dependent, in this work we deepen the study of the mechanism of action of HCB on VF in the EA-hy926 endothelial line, grown in DMEM, 10% FBS, at 37 ° C treated with HCB (0.05, 0.05 and 5μM) at different times (18 and 24 hours). We analyze: a-Molecules involved in contractility and BP , TGF-β1 and RE-α [Western blot (W), RT-PCR]. b-Levels of eNOs and nitrites (W, Greiss). c-Role of TGF-β1 in the expression of RE-α (TGF-R RII inhibitor, SB431542). d-Rol of thyroid hormones (TH) in the expression of TGF-β1 and RE-α [serum depleted TH, exogenous T3 (10-7M)], since HCB is an endocrine disruptor and induces hypothyroxinemia, and that TH regulates the expression of these parameters.a-T TGF-β1 (protein) increased 24%, (p