Cobalt chloride acts a cardioprotector after an ischemia/reperfusionEPERFUSION INJURY IN INFANT RATS.

GUTIERREZ, CHRISTOPHER P. A.; PEIRONE, MAGDALENA; LITTLE , TOMÁS; RAMIREZ, MATIAS; BONAZZOLA, PATRICIA; CASTILLA, ROCÍO

Mar del Plata

Congreso; Reunión Anual de las Sociedades de Biociencia 2023.; 2023

Sociedad Argentina de Investigación Clínica

The damage induced by Ischemia-reperfusion (I/R) involves alterations in Ca2+ homeostasis and is reduced by ischemic postconditioning. CoCl2 can trigger changes resembling the response to a hypoxic event in normoxia and blocks Ca2+ current in heart muscle. Previously we have demonstrated in adult rat heart that CoCl2 administrated in reperfusion can reduce damage induced by I/R consistent with improved cellular calcium handling.Since the hearts of infant rats differ from those of adults in the dependence on plasma Ca2+ for myocyte function, we evaluated whether CoCl2 can also protect infant heart from I/R damage.For that proposal, ventricles from 6-8 days old Wistar rats were isolated, arterially perfused, suspended and connected to an isometric force transducer. Ventricles were introduced in an organ bath at 37 °C, stimulated at 3Hz and subjected or not to 45 min of ischemia followed by reperfusion in the presence of 0.23 mmol/L CoCl2. Mechanic parameters were analyzed.The presence of CoCl2 increased post ischemic contractile recovery (developed tension at 45 min: control: 26.4 ± 1.7 %, CoCl2: 51.8 ± 8.3 % p