Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi

ROCHA, C. M. L.;; VELLICCE, G. R.; ; GARCÍA, M. G.; ; PARDO, E. M.; .; RACEDO, J.; ; PERERA, M. F.;; DE LUCÍA, A. D.; ; GILLI, J.; ; BOGADO, N.; ; BONECARRÉRE, V.; ; GERMAN, S.; ; MARCELINO, F. C.; ; LEDESMA, F.; ; PLOPER, L. D.;; WELIN, B.; CASTAGNARO, A. P

ELECTRONIC JOURNAL OF BIOTECHNOLOGY

UNIV CATOLICA DE VALPARAISO

Lugar: Valparaíso; Año: 2015

0717-3458

Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the maindiseases affecting soybean and has been reported as one of the most economically important fungalpathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered whendeveloping resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizicombining simple sampling with a powerful and reproducible molecular technique.Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for theamplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 productionfields. From a total of 1,919 markers obtained, 77% were polymorphic. The high percentage ofpolymorphism and the Nei´s genetic diversity coefficient (0.22) indicated high pathogen diversity.Analysis of Molecular Variance showed higher genetic variation within countries than among them.Temporal analysis showed a higher genetic variation within a year than between years. Cluster,Phylogenetic and Principal Co-ordinate analysis showed that samples group by year of collection andthen by country sampled.Conclusions: The study proposed combining a simple collection of urediniospore with a subsequentanalysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizicollected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLPanalysis is an important and potent molecular tool for the study of genetic diversity and could be usefulto carry out wider genetic diversity studies.