INVESTIGADORES
CASTAGNARO Atilio Pedro
artículos
Título:
Biofilm formation, epiphytic fitness and canker development in Xanthomonas axonopodis pv. citri.
Autor/es:
LUCIANO RIGANO, FLORENCIA SICILIANO, RAMÓN ENRIQUE, LORENA SENDÍN, PAULA FILIPPONE, PABLO TORRES, JULIA QÜESTA, MAX DOW, ATILIO PEDRO CASTAGNARO, ADRIÁN VOJNOV AND MARÍA ROSA MARANO
Revista:
MOLECULAR PLANT-MICROBE INTERACTIONS
Referencias:
Año: 2007 vol. 20 p. 1222 - 1230
ISSN:
0894-0282
Resumen:
The phytopathogenic bacterium Xanthomonas axonopodisXanthomonas axonopodis
pv. citri is responsible for the canker disease affecting citrus
plants throughout the world. Here, we have evaluated
the role of bacterial attachment and biofilm formation in
leaf colonization during canker development on lemon
leaves. Crystal violet staining and confocal laser scanning
microscopy analysis of X. axonopodis pv. citri strains expressing
the green fluorescent protein were used to evaluate
attachment and biofilm formation on abiotic and biotic
(leaf) surfaces. Wild-type X. axonopodis pv. citri attached to
and formed a complex, structured biofilm on glass in minimal
medium containing glucose. Similar attachment and
structured biofilm formation also were seen on lemon
leaves. An X. axonopodis pv. citri gumB mutant strain, defective
in production of the extracellular polysaccharide
xanthan, did not form a structured biofilm on either
abiotic or biotic surfaces. In addition, the X. axonopodis pv.citri is responsible for the canker disease affecting citrus
plants throughout the world. Here, we have evaluated
the role of bacterial attachment and biofilm formation in
leaf colonization during canker development on lemon
leaves. Crystal violet staining and confocal laser scanning
microscopy analysis of X. axonopodis pv. citri strains expressing
the green fluorescent protein were used to evaluate
attachment and biofilm formation on abiotic and biotic
(leaf) surfaces. Wild-type X. axonopodis pv. citri attached to
and formed a complex, structured biofilm on glass in minimal
medium containing glucose. Similar attachment and
structured biofilm formation also were seen on lemon
leaves. An X. axonopodis pv. citri gumB mutant strain, defective
in production of the extracellular polysaccharide
xanthan, did not form a structured biofilm on either
abiotic or biotic surfaces. In addition, the X. axonopodis pv.X. axonopodis pv. citri strains expressing
the green fluorescent protein were used to evaluate
attachment and biofilm formation on abiotic and biotic
(leaf) surfaces. Wild-type X. axonopodis pv. citri attached to
and formed a complex, structured biofilm on glass in minimal
medium containing glucose. Similar attachment and
structured biofilm formation also were seen on lemon
leaves. An X. axonopodis pv. citri gumB mutant strain, defective
in production of the extracellular polysaccharide
xanthan, did not form a structured biofilm on either
abiotic or biotic surfaces. In addition, the X. axonopodis pv.X. axonopodis pv. citri attached to
and formed a complex, structured biofilm on glass in minimal
medium containing glucose. Similar attachment and
structured biofilm formation also were seen on lemon
leaves. An X. axonopodis pv. citri gumB mutant strain, defective
in production of the extracellular polysaccharide
xanthan, did not form a structured biofilm on either
abiotic or biotic surfaces. In addition, the X. axonopodis pv.X. axonopodis pv. citri gumB mutant strain, defective
in production of the extracellular polysaccharide
xanthan, did not form a structured biofilm on either
abiotic or biotic surfaces. In addition, the X. axonopodis pv.X. axonopodis pv.
citri gumB showed reduced growth and survival on leaf
surfaces and reduced disease symptoms. These findings
suggest an important role for formation of biofilms in the
epiphytic survival of X. axonopodis pv. citri prior to development
of canker disease.showed reduced growth and survival on leaf
surfaces and reduced disease symptoms. These findings
suggest an important role for formation of biofilms in the
epiphytic survival of X. axonopodis pv. citri prior to development
of canker disease.X. axonopodis pv. citri prior to development
of canker disease.