Isolation and purification of edaphic Cyanobacteria in the province of San Luis (Argentina).

CHIOFALO, SERGIO; FERNANDEZ BELMONTE, CECILIA; CASSÁN, FABRICIO

Mendoza

Congreso; XXXVI Reunión anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Cyanobacteria applied as bio-fertilizers improve soil properties and promote plant growth. To assess these skills individually and by species, it is necessary to isolate and purify them. The aim of this work was to obtain unialgal crops of native soil cyanobacteria for subsequent agronomic testing. Sampling was carried out in agricultural-livestock lots of corn, soybeans and alfalfa, 25 km north of Villa Mercedes, on July 2014. Soil samples were taken from 0 to 10 cm deep and placed in Petri dish with Ripkka liquid culture medium and were put into a growth chamber at 25-30 ° C with a photoperiod of 12 hours light ? 12 hours darkness. After a month, algal biomass was extracted and microscopically identified. The species of agronomic interest were chimed in Petri dishes with Rippka agarized medium at 1%. Depending on the mobility of the species in the agarized medium two forms of chimes were made in order to collect their edges with greater purity - one in the form of rifling for species with low mobility and the other in the form of islands for species with greater mobility. Each chime delayed its growth between 10 to 15 days. 12 to 15 chimes were performed and purity was microscopically tested to confirm each unialgal crop. Then, chime in test tube with Rippka liquid medium with Cycloheximide 100 mg / l was made for 15 days in order to eliminate contaminating fungi. Subsequently, chime was made in Erlenmeyer flasks with Ripkka liquid medium and the samples were cultivated with periodic agitation for biomass growth. Isolated and multiplied species of agronomic interest. were Calothrix sp, Nostoc muscorum, Nostoc calcicola, Nostoc sp, Anabaena oryzae, Anabaena sp. Genera and some species of cyanobacteria obtained are consistent with those isolated in South Africa, Italy, India, Pakitan, Czech Republic and Egypt, where they had good results as biofertilizer. Therefore, they isolate obtained in this study could have future applications such as bio-fertilizers. Isolation unialgal through island shaped chimes on petri dish is a useful method to cyanobacteria of fast growth.