The alfalfa silage conservation as alternative for agronomic use of soil and plant-associated bacteria

CRISTIANI, MARIANA; MASSERA, ARIEL; CASSÁN, FABRICIO; GAGGIOTTI MONICA; ROMERO, LUIS

Medellín

Congreso; 9th International PGPR and First Latinamerican PGPR Workshop; 2012

PGPR Workshop Comitte

Silage conservation is strategic practice in agriculture and livestock in Argentina. Ensilage is a preservation method in which lactic acid bacteria (LAB) from plant, soil or those introduced by inoculation, metabolizes soluble carbohydrates into organic compounds, as lactic acid with a significant pH reduction in environment. LAB are considered a real biocontrol bacteria, because they can inhibit growth of wide broad of organisms, including many phytopathogens during process. The aim of this study was to evaluate and compare the microbiological fitness of plant native and introduced LAB, and its effect on fermentation and quality of alfalfa silage. We evaluated the quality and characteristic of three commercial inoculant based on complex consortium of LABby counting and then identification (still running) in selective culture medium [MRS]. According this, only one inoculant was recommendedfor further microsilage assays [5 kg] because contains more than 1E+09 ufc.ml-1 of declared Lactobacillus (3 species), Pediococus, Enterococus and other LAB. Alfalfa plants were harvested in January 2012 by use of conventional technology for this legume. The experiment consistedof three repetitions of the following treatments: (1) not inoculated; (2) inoculated with simple dose of product previously selected, and (3) inoculatedwith double dose of the same product. We evaluated ensilage quality control parameters and the number and type (still running) of LAB along time. Our results on alfalfa ensilage quality parameters and microbial evolution will be presented in detail in the poster.