Genomic analysis of Bradyrhizobium japonicum E109 a soybean-symbiotic strain which display a wide broad of plant growth promoting mechanisms.

CASSÁN, FABRICIO; REVALE, SANTIAGO; MANCINI, ESTEFANIA; VASQUEZ, MARTIN; SPAEPEN, STIJN; VANDERLEYDEN, JOS; PERTICARI, ALEJANDRO

Medellín

Congreso; 9th International PGPR and First Latinamerican PGPR Workshop; 2012

PGPR Workshop Comitte

B. japonicum E109 was selected during 1980s by IMYZA-INTA as the most suitable strain for soybean inoculation in Argentina. Numerous laboratory, greenhouse and field experiments since then have demonstrated the ability of E109 to increase soybean productivity under diverse agronomical conditions by the atmospheric nitrogen fixation mechanism. Furthermore, there are increasing reports on E109 capacity to promote growth in non-legumes and other crops by occurrence of a wide broad of mechanisms different to BNF. In this work, we present a comprehensive analysis of the genomic features and plant growth promotion mechanisms, besides to the well studied symbiotic-nitrogen fixation. The genome sequence was obtained using a Whole Genome Shotgun strategy with a 454 GS Titanium pyrosequencer at INDEAR. Assembly was done using 454 Newbler v2.6 with a 24X genome coverage. A total of 119 large contigs were obtained. The draft genome was 9,148,167 bases in length with a mean GC content of 63.7%. Genome annotation was done using the Standard Operating Procedures for Prokaryotic annotation from ISGA and from RAST annotation server. A total of 8,747 Coding Sequences (CDS) and 53 structural RNAs were predicted. Annotation covered 529 RAST subsystems with 3,095 CDS (36%), while 3,838 (44%) were classified as hypothetical proteins. The putative plant growth promotion mechanisms of E109 will be discussed in the complete presentation.