Azospirillum spp. Metabolize [2H2]gibberellin A20 to [2H2]gibberellin A1 in vivo in dy Rice Mutant Seedlings

FABRICIO D. CASSÁN, CARLOS D. LUCANGELI, RUBÉN BOTTINI AND PATRICIA N. PICCOLI

PLANT AND CELL PHYSIOLOGY

Oxford journal

Lugar: Oxfordshire; Año: 2001 vol. 42 p. 763 - 767

0032-0781

Azospirillum spp. are endophytic bacteria with beneficial effects on cereals-effects partially attributed to gibberellin production by the microorganisms. Azospirillum lipoferum and Azospirillum brasilense inoculated to rice dy mutant reversed dwarfism in seedlings incubated with [17,17-2H2]GA20 with formation of [17,17-2H2]GA1, showing the in vivo capacity to perform the 3ß-hydroxylation. When Prohexadione-Ca, an inhibitor of late steps in gibberellin biosynthesis, was added to the culture medium, no complementation was observed and no [17,17-2H2]GA1 was produced. The latter suggests that the bacterial operating enzyme may be a 2-oxoglutarate-dependent dioxygenase, similar to those of plants.