INVESTIGADORES
CASSAN Fabricio Dario
artículos
Título:
Genotypic characterization of azotobacteria isolated from Argentinean soils and plant-growth-promoting traits of selected strains with prospects for biofertilizer production
Autor/es:
RUBIO, ESTEBAN; MONTECHIA, MARCELA; CASSAN, FABRICIO; PERTICARI, ALEJANDRO; CORREA, OLGA
Revista:
SCIENTIFIC WORLD JOURNAL, THE
Editorial:
THESCIENTIFICWORLD LTD
Referencias:
Lugar: New York; Año: 2013 p. 1 - 12
ISSN:
1537-744X
Resumen:
The genetic diversity among 31 putative
Azotobacter isolates obtained from agricultural and non-agricultural soils was
assessed using rep-PCR genomic fingerprinting and identified to species level
byARDRA and partial 16S rRNAgene sequence analysis. High diversity was found
among the isolates, identified as A. chroococcum, A. salinestris, and A.
armeniacus. Selected isolates were characterized on the basis of phytohormone
biosynthesis, nitrogenase activity, siderophore production, and phosphate
solubilization. Indole-3 acetic-acid (IAA), gibberellin (GA3) and zeatin (Z)
biosynthesis, nitrogenase activity, and siderophore production were found in
all evaluated strains, with variation among them, but no phosphate
solubilization was detected. Phytohormones excreted to the culture medium
ranged in the following concentrations: 2.2-18.2 ug IAAmL−1, 0.3-0.7 ug
GA3 mL−1, and 0.5-1.2 ugZmL−1. Seed inoculations with further selected
Azotobacter strains and treatments with their cell-fre cultures increased
the number of seminal roots and root hairs in wheat seedlings. This latter
effect was mimicked by treatment with IAA-pure solutions, but it was not
related to bacterial root colonization. Our survey constitutes a first approach
to the knowledge of Azotobacter species inhabiting Argentinean soils in three
contrasting geographical regions. Moreover, this phenotypic characterization
constitutes an important contribution to the selection of Azotobacter strains
for biofertilizer formulations.