Sulfanilic acid-modified chitosan mini-spheres and their application for lysozyme purification from egg white

HIRSCH, DANIELA B.; BAIELI, MARIA F.; URTASUN, NICOLÁS; LÁZARO-MARTÍNEZ, J.M.; GLISONI, ROMINA J.; MIRANDA, MARÍA V.; CASCONE, OSVALDO; WOLMAN, FEDERICO J.

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Año: 2018 vol. 34 p. 387 - 396

8756-7938

A cation exchange matrix with zwitterionic and multi-modal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico-chemically characterized by ss-NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g-1 while the dissociation constant was 0.074 ± 0.012 mg mL-1. The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP-HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials.