INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Partitioning and purification of thaumatin in aqueous two-phase systems
Autor/es:
O. CASCONE; B.A. ANDREWS; J.A. ASENJO
Revista:
ENZYME AND MICROBIAL TECHNOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 1991 vol. 13 p. 629 - 635
ISSN:
0141-0229
Resumen:
The partition behaviour of pure thaumatin in aqueous two-phase systems has been examined in order to investigate the effects of changes in phase components on the partitíon coefficient, K. Factors such as MW of PEG, pH, and concentration of NaCl (and other salts) were all found to influence K, whereas the protein concentration had no effect (up to 40 g/l under optimum partitíoning conditíons). Conditions were found in PEG/phosphate systems where the partition coefficient, K, can be increased 60 times from K = 0.53 to K = 33. The separation and purification of thaumatin from a typical protein contaminant such as those in a homogenate of E. coli ce1ls was studíed. Optimum conditions were found by studying partítion conditions of the thaumatin and E. coli homogenate proteins independently. Such conditions were then used and investigated with a mixture of thaumatin and E. coli homogenate proteins using a reversed phase HPLC method to follow the concentration of thaumatin in the protein mixture.
