Foot and mouth disease virus purification by affinity chromatography

A.A. NAVARRO DEL CAÑIZO; M. MAZZA; R. BELLINZONI; O. CASCONE

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

HUMANA PRESS INC

Año: 1996 vol. 61 p. 399 - 409

0273-2289

Foot and mouth disease virus, (FMDV) from a crude cell lysate was purified in a single step by affinity chromatography with heparin as a ligand. The virus eluted from an Heparin-Ultrogel A4R column at 1 M sodium chloride in 10 mM sodium phosphate buffer, pH 7.0, while most cell protein and albumin did so at lower concentrations of sodium chloride in the same buffer. Purity of the eluted fraction containing the virus was assessed by SDS-PAGE, HPLC, ultracentrifugation and UV absorption spectrum. With this method, intact viral particles are recovered in high yield (over 90%)and specific virus purity increases nearly 1000-fold. The capacitiy of the chromatographic matrix for the virus was found to be 1.1 mg viral mass per mL of hydrated gel.