INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Assessment of different treatments for the concentration of three tomato fruit glycosidases from crude extracts
Autor/es:
G.O. SOZZI; S.A. CAMPERI; A.A. FRASCHINA; O. CASCONE
Revista:
BIOTECHNOLOGY TECHNIQUES
Editorial:
SPRINGER
Referencias:
Año: 1998 vol. 12 p. 645 - 648
ISSN:
0951-208X
Resumen:
Ultrafiltration, (NH)rSOoaddition to 80% saturation and lyophilization after desalting, were assessed as different methods for the concentration of tomato fruit a- and B-galactosidases and s-L-arabinofuranosidase. Lyophilization was the most effective giving 95%,82% and 75%o recovery of enzyme activities, respectively. These enzymes are stable when preserved after lyophilization or in concentrated crude extracts at 20 or 50°C even after extended storage, either in the presence or absence of trehalose.