INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Acid protease purification by dye affinity chromatography
Autor/es:
A.A. NAVARRO DEL CAÑIZO; H.M. FERNÁNDEZ LAHORE; M.V. MIRANDA; O. CASCONE
Revista:
AFINIDAD
Editorial:
IQS
Referencias:
Lugar: Barcelona; Año: 2001 vol. 58 p. 231 - 233
ISSN:
0001-9704
Resumen:
After screening of 9 immobilised triazinic dyes, Red F5B was selected as the best ligand for chymosin and Mucor miehei acid protease purification, at pHs 6.5 and 5.4 respectively. For Mucor bacilliformis protease purification from a crude extract of a solid-state culture, Red HE-3B was selected, allowing a 95.2% recovery of pure enzyme.
