INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Rapid affinity purification processes for CGTase from Bacillus circulans
Autor/es:
A. ROSSO; S. FERRAROTTI; M.V. MIRANDA; N. KRYMKIEWICZ; B.C. NUDEL; O. CASCONE
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
Springer
Referencias:
Año: 2005 vol. 27 p. 1171 - 1175
ISSN:
0141-5492
Resumen:
Two rapid and easy-to-scale-up methods for Bacillus circulans DF9R cyclodextrin glycosyltransferase (CGTase) purification were developed: affinity precipitation with starch and aqueous two-phase partition. The first method was optimised by a factorial design, allowing an 80 % CGTase adsorption at 11 % starch and 1.6 % ammonium sulphate, and a 65 % recovery after elution with 10 mM a-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72 % CGTase recovery in a two-step procedure, CGTase was obtained in the bottom phase with a purification factor of 37.
