Immobilisation of soybean seed coat peroxidase on its natural support for phenol removal from wastewater

MAGRI, MARÍA L.; LOUSTAU, M. N.; V. MIRANDA, MARIA; OSVALDO CASCONE

BIOCATALYSIS AND BIOTRANSFORMATION

Informa Healthcare

Año: 2007 vol. 25 p. 1 - 5

1024-2422

  Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier. Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier. Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier. Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier. Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier. Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier. Soybean seed coat peroxidase (SBP, EC 1.11.1.7) was immobilised on its natural support, soybean seed coats, anticipating its use in phenol removal. Periodate and glutaraldehyde chemistries were assayed. Periodate failed to immobilise any amount of SBP whereas glutaraldehyde was effective. The optimum concentration of glutaraldehyde was found 1%. Immobilisation shifted enzyme optimum pH for phenol removal from 4.0 to 6.0. Treated seed coat conserved its activity over a four-week period. A reusability assay showed that treated seed coats could be reused once for phenol removal. PEG stabilised phenol degradation activity in the course of time. Besides, the phenolic polymer was adsorbed on seed coats thus making removal of the polymeric product easier.