INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Trinitrophenylation of bovine growth hormone
Autor/es:
M.J. BISCOGLIO DE JIMÉNEZ BONINO; O. CASCONE; J.A. SANTOMÉ
Revista:
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH
Editorial:
Munskgaard
Referencias:
Año: 1979 vol. 14 p. 107 - 112
ISSN:
0367-8377
Resumen:
Bovine growth hormone was chemically modified wíth picryl sulfonic acid, at pH 8.4 during 2 and 5 min of reaction. The N-terminal residue provídes the most reactive amino group followed by the ?-amíno groups of lysine 179 and lysines 143, 69, 111, 170 and 166 in decreasing order. These results agree with those obtained previously wíth equíne growth hormone, except that residue 156 is not modifíed in bovine growth hormone. An important decrease in biological activity occurs between 2 and 5 min of reaction wlthout sensible modification in the alpha-helix content of the molecule.
