INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Oxidation of methionine residues in bovine growth hormone by Chloramine-T
Autor/es:
O. CASCONE; M.J. BISCOGLIO DE JIMÉNEZ BONINO; J.A. SANTOMÉ
Revista:
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH
Editorial:
Munskgaard
Referencias:
Año: 1980 vol. 16 p. 299 - 305
ISSN:
0367-8377
Resumen:
The methioníne residues in bovine growth hormone were chemically modified with chloromine-T at pH 7.4 using dífferent chloramíne-T methioníne molar ratíos. Methionine 4 was shown to be the most reactive followed ín decreasing order by methionines 148, 123 and 178. With a 50-fold molar excess of chloramine-T over methionine almost full oxidation of all the methionine residues was achieved. The oxidation of the four residues of methionine does not change the growth promoting activity of the hormone.
