INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Isolation and characterization of alpaca growth hormone
Autor/es:
M.J. BISCOGLIO DE JIMÉNEZ BONINO; O. CASCONE; A.I. ARNAO DE NUÉ; J.A. SANTOMÉ; D. SÁNCHEZ; R. ORÉ; M. VILLAVICENCIO
Revista:
INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH
Editorial:
Munskgaard
Referencias:
Año: 1981 vol. 17 p. 374 - 379
ISSN:
0367-8377
Resumen:
A simple and mild procedure is described for the isolation of pituitary growth hormone from alpaca glands. It involves extractions in buffer at pH 8.7 and gel filtration in Sephadex G-75. An average yield of l mg purífied product may be obtained from 1 g fresh alpaca pituitary glands. The alpaca hormone was examined for homogeneity by SDS-polyacrilamide gel electrophoresis, gel electrofocusing and end-group analyses. It is a protein composed of a single polypeptide chain with phenylalanine at both ends. The C-terninal sequence is -Ala-Phe. The molecular weight is approximately 21700 and the amino acíd composition very similar to that observed in equine growth hormone.
