Basic and acidic hydrophilic residues involved in the interaction between protomers of the bovine growth hormone dimer

J.G. FUKUSHIMA; J.M. DELFINO; C.E.M. WOLFENSTEIN-TODEL; M.J. BISCOGLIO DE JIMÉNEZ BONINO; O. CASCONE; H.N. FERNÁNDEZ; J.A. SANTOMÉ

Acta Physiologica et Pharmacologica Latinoamericana

Asociación Latinoamericana de Ciencias Fisiológicas

Año: 1984 vol. 34 p. 151 - 156

0326-6656

Reactivity of histidine and arginine residues as well as of carboxyl groups in the covalently stabilized dimer of bovine growth hormone, was studied in comparison with their reactivity in the monomeric form. Results obtained by reaction of histidine and arginine residues with ethoxyformic anhydride and cyclohexanedione, respectively, were similar for both proteins. The reactivity of te carboxyl groups towards a soluble carbodiimide becomes impaired in the dimer, thus suggesting that their location is within the protomer interaction area.