INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Hydrophobicity modulation as a tool for the purification of histidyl peptides
Autor/es:
M.J. BISCOGLIO DE JIMÉNEZ BONINO; J.G. FUKUSHIMA; O. CASCONE
Revista:
ANALYTICAL BIOCHEMISTRY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 1986 vol. 157 p. 8 - 11
ISSN:
0003-2697
Resumen:
A methodology is described for purification of histidyl peptides based on the changes in hydrophobicity induced by the speciñc and reversible modification of histidine residues by ethoxyformic anhydride. The mixture of modified peptides is subjected to HPLC: peptides containing
modified histidines are detected at 242 nm, recovered, deethoxyformylated and rechromatographed under the same conditions, then being detected at 220 nm. This procedure allows their isolation free from contaminants.
