Direct lysozyme separation from egg-white by dye membrane affinity chromatography

M. GRASSELLI; S.A. CAMPERI; A.A. NAVARRO DEL CAÑIZO; O. CASCONE

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

Wiley Interscience

Año: 1999 vol. 79 p. 333 - 339

0022-5142

  An affinity membrane from hydrophylized polyethylene hollow fibre as the support matrix was prepared. Red HE-3B was immobilised on the membrane and the adsorption behaviour of lysozyme solutions and homogenized egg white in the membranes was investigated. Dye density (1.7 µmol mL-1) and maximum binding capacity (26 mg lysozyme) are comparable to those of commercial gel matrices. Dynamic binding capacity did not change when residence time was decreased from 2.57 s to 1.03 s or with lysozyme concentrations from 0.043 to 0.057 mg mL-1 while, at 0.025 mg mL-1 feed concentration, breakthrough was poorer. A method for direct lysozyme separation from egg white was developed, having a productivity of 6 Kg lysozyme/ m3. h. The purity of the eluted lysozyme, as determined by HPLC, was 85 %, with a recovery of 92 %. Dynamic capacity was kept constant at 70 % of the maximum binding capacity for at least 10 cycles through membrane regeneration with 0.1 M NaOH - 1 M NaCl.