INV SUPERIOR JUBILADO
CASCONE Osvaldo
artículos
Título:
Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries
Autor/es:
S.A. CAMPERI; N.B. IANNUCCI; G.J. ALBANESI; M. OGGERO EBERHARDT; M. ETCHEVERRIGARAY; F. ALBERICIO; A. MESSEGUER; O. CASCONE
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
Springer
Referencias:
Año: 2003 vol. 25 p. 1545 - 1548
ISSN:
0141-5492
Resumen:
Peptide Ala-Pro-Ala-Arg (APAR) was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 µmol/ml, showed a maximum capacity of 9.1 mg Mab/ml and a dynamic capacity of 3.9 mg Mab/ml. 95 % yield of electrophoretically pure anti-GM-CSF was obtained in a single step.
