Improved hollow-fibre membranes for dye-affinity chromatography

F.J. WOLMAN; E.E. SMOLKO; O. CASCONE; M. GRASSELLI,

JOURNAL OF SEPARATION SCIENCE - (Online)

Wiley

Lugar: Weinheim; Año: 2005 vol. 28 p. 45 - 51

1615-9314

              Hollow-fibre membranes with a different surface hydrophilicity degree were obtained by grafting mixtures of  glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G-A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity increased with the amount of dimethyl acrylamide in the grafted polymer. As hydrophilicity increased permeability decreased from 352 ml/cm2.min.MPa for membranes grafted with GMA with ammonia spacer to 12.7 ml/cm2.min.MPa for membranes grafted with GMA/DMAA 1/3 with glucamine spacer. Membranes grafted with GMA/DMAA 1/3 with ammonia spacer showed the best performance for BSA and lysozyme adsorption: maximum capacity was 15.3 ± 2.2 mg BSA/ml membrane and 58.3 ± 6.6 mg lysozyme/ml membrane while dissociation constants were 0.27 ± 0.16 and 0.13 ± 0.12 mg/ml, respectively. Over 80% of adsorbed proteins could be eluted with 2 M NaCl + 20% isopropanol in 20 mM sodium phosphate buffer, pH 7.0.