CAMPERI Silvia Andrea
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
S. L. SAAVEDRA; M. C. MARTÍNEZ CERON; S. L. GIUDICESSI; G. FORNO; M. B .BOSCO; M. M. MARANI; R. ERRA-BALSELLS; F. ALBERICIO; O. CASCONE; S.A. CAMPERI
AMER CHEMICAL SOC
Lugar: Washington; Año: 2018 vol. 34 p. 999 - 999
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied its ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 90% yield and 90% purity - as judged by SDS-PAGE - in a single step with a purification factor of 4.6.