Post-column in-source derivatization in LCMS: a tool for natural products characterization and metabolomics

ADRIANA M. CIRIGLIANO; GABRIELA M. CABRERA

Conferencia; International Mass Spectrometry Conference 2018; 2018

Metabolite characterization is crucial for many aspects of basic research,such as de-replication of crude extracts in natural products chemistryor tentative identification in untargeted metabolomics. This information makes the subsequent decision-making process easier and more efficient. Derivatization with hydrazines is a known method in this respect, which allows the characterization of carbonyl functional groups [1-2].In this work, we report the use of post column derivatization withhydrazines of carbonylic groups from triterpenoids like cycloartanes, steroids,cardenolides and other terpenoids using HPLC-MS in a QTOF.