Statistical optimisation of the exopolysaccharide production by Lactobacillus fermentum Lf2 and analysis of its chemical composition.

ALE, E.; BATISTELA, V.; CORREA OLILVAR, G.; FERRADO, J.; SADIQ, S.; AHMED, H.; REINHEIMER, J. A.; VERA CANDIOTI, L.; LAWS, A.; BINETTI, A. G.

INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2019

1364-727X

Lactobacillus fermentum Lf2produces high amounts of exopolysaccharides (EPS) (~1 g/L) with demonstrated functionaland technological roles when applied as a food ingredient in dairy matrices, propertiesthat made these EPS interesting in comparison with other similar molecules fromlactic acid bacteria (LAB). Those characteristics encouraged us to optimise theproduction. The EPS extract is composed of a high molecular mass β-glucan and amedium molecular mass heteroglycan. In the present work, the optimal conditionsthat doubled the EPS yield using a semi-defined medium (SDM, 0.63% yeastnitrogen base, 0.53% bacto casitone, 0.53% ammonium citrate, 6.25% sucrose, pH6.5) were found by means of response surface methodology (RSM). The chemicalcharacterization indicated that under optimised conditions the synthesis of theheteroglycan was favoured compared with that of the β-glucan.Keywords: exopolysaccharides; statisticaloptimisation; chemical composition, L.fermentum; response surface methodology.