delta-Aminolevulinic Acid Transport through Blood Brain Barrier

CORREA GARCIA SUSANA; BERMÚDEZ-MORETTI, MARIANA; ROUVIER GARAY, VERÓNICA; BATLLE ALCIRA

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM

PERGAMON

Lugar: Oxford; Año: 1998 vol. 31 p. 579 - 582

0306-3623

ABSTRACT. 1. d-Aminolevulinic acid (ALA) and g-aminobutyric acid (GABA) transport through the rat blood±brain barrier (BBB) was studied.2. GABA transport (Km 53.260.1 mM and V 50.29960.045 mmol/mg protein min) is probably mediated by a permease similar to Saccharomyces cerevisise PUT4; this result also indicates that the isolated capillaries are metabolically active.max3. ALA incorporation into rat brain endothelial cells is not detected even in capillaries treated to expose the luminal surface.4. Previous evidence indicates that ALA can cross the BBB from blood to brain; so we conclude that, with the methodology used here, transport in the luminal membrane of the capillaries cannot be investigated.