FTIR spectroscopy of chronic venous leg ulcer exudates: an approach to spectral healing marker identification.

NICOLÁS CERÚSICO; JUAN PABLO AYBAR; SILVANA LÓPEZ; SILVIA G. MOLINA; ROMINA CHAVEZ JARA; EUGENIA SESTO CABRAL; JUAN CARLOS VALDEZ; AIDA BEN ALTABEF; ALBERTO N. RAMOS

ANALYST

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2018 vol. 143 p. 1583 - 1592

0003-2654

Chronic venous leg ulcer (CVLU) arises as a chronic venous insufficiencycomplication and is a major cause of morbidity throughout the world.Ourhypothesis is that CVLU exudatecomposition is a biochemical representation of wound clinical state. Then,Fourier Transform Infrared (FTIR) spectroscopy could be a useful andless-invasive technique to study the clinical state of the ulcer. For this, the aim of this work was to perform a spectralcharacterization of exudate from CVLU using FTIR spectroscopy to identifypotential healing markers.45 exudate samples from CVLU, 95% of the strains isolated from CVLU inplanktonic and biofilm phenotypes and other related biological samples as humanplasma, serum, urine, blood cells, urea, creatinine, glucose and albumin werestudied by FTIR spectroscopy. According to vibration frequency of biomolecules(lipids, protein, nucleic acids and carbohydrates) characteristic bonds atinfrared region, different spectral windows were selected and spectral areas ofeach window were measured. Besides, Savitzky-Golay second derivative wereobtained for all spectra and peaks from each standardized window were detected.FTIR spectroscopy allowed to identify sample types (exudate, plasma, serum,urine) as each one present a unique relative composition and ratios ranges.Also, this technique could be useful to identify bacteria at phenotypic-ulcerstate and allows to differentiate if bacteria are in biofilm or planktonic formwhich is unlikely by conventional methods.In this work we found some spectral markers (areas, peaks) that allow toidentify several parameters in exudate as:a) Total cellularity, b) Inflammatorycells load, c) Bacterial load, d) Fibrin amount, e) Inflammatoryproteins.Because the measured areas or founded peaks areconcentration-dependent this method could also serve to measure them.Therefore, FTIR spectroscopy could be useful to evaluate patientevolution as all these exudate parameters represent critical negative markersfor wound healing.