Vibrational Analysis of the Hydrogen Bonding of Cytidine and Guanosine Derivatives

P. CARMONA; M. MOLINA; A. LASAGABASTER; R. ESCOBAR; A. BEN ALTABEF

JOURNAL OF PHYSICAL CHEMISTRY

AMER CHEMICAL SOC

Año: 1993 vol. 97 p. 9519 - 9524

0022-3654

Hydrogen bonding between 2’-deoxy-3’,5’-bis(triisopropylsilyl)guanosine (G) and 2’-deoxy-3’,5’-bis(triisopropylsily1) cytidine (C) has been studied by vibrational spectroscopy in chloroform solution. Strong interactions occur between the two derivatives of guanosine and cytidine and between CG base pair and cytidine, whose association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band occur between the two derivatives of guanosine and cytidine and between CG base pair and cytidine, whose association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band occur between the two derivatives of guanosine and cytidine and between CG base pair and cytidine, whose association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band cytidine (C) has been studied by vibrational spectroscopy in chloroform solution. Strong interactions occur between the two derivatives of guanosine and cytidine and between CG base pair and cytidine, whose association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band occur between the two derivatives of guanosine and cytidine and between CG base pair and cytidine, whose association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band occur between the two derivatives of guanosine and cytidine and between CG base pair and cytidine, whose association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band acceptors of both nucleobases and the guanine N(2)H and cytosine N(4)H donors. The main spectral changes of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band of CG dimer accompanying CGC trimer formation are intensity and frequency decreases of the Raman band association constants were first determined. CGC trimers involve cyclic hydrogen bonds through the N(3) acceptors