INVESTIGADORES
BELTRAMO Dante Miguel
artículos
Título:
Superstimulation of ovarian follicular development in beef cattle with a single intramuscular injection of Folltropin-V
Autor/es:
ANDRÉS TRÍBULO, DRAGAN ROGAN, HUMBERTO TRIBULO, RICARDO TRIBULO, ROXANA V.ALASINO,B, DANTE BELTRAMO, ISMAEL BIANCO, REUBEN J. MAPLETOFT, GABRIEL A. BÓ
Revista:
ANIMAL REPRODUCTION SCIENCE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011
ISSN:
0378-4320
Resumen:
The need to inject FSH twice daily for superstimulation of ovarian follicular development in
24 cattle necessitates frequent attention by farm-personnel and increases the possibility of failures
25 due to mishandling and errors in administration of treatments. A series of three experiments were
26 designed to evaluate the feasibility of superstimulation in beef cattle with a single intramuscular
27 (IM) injection of Folltropin-V diluted in a hyaluronan-based slow-release formulation (SRF). In
28 Experiment l, cows were assigned to one of three treatment groups to compare two methods of
29 injection as compared to the twice daily IM injection protocol. Superovulatory response of cows
30 (n = 6) treated with twice daily IM injections over 4 d (Control) was greater than of cows treated
31 with a single subcutaneous (SC) injection in SRF (n = 6), while superovulatory response of cows
32 treated with a single IM injection in SRF (n = 6) was intermediate. Experiment 2 was designed to
33 compare two concentrations of SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL
34 hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL)
35 were not significantly different (P ¡Ý 0.05), but the numbers of total ova/embryos (P < 0.05),
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
33 compare two concentrations of SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL
34 hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL)
35 were not significantly different (P ¡Ý 0.05), but the numbers of total ova/embryos (P < 0.05),
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
32 treated with a single IM injection in SRF (n = 6) was intermediate. Experiment 2 was designed to
33 compare two concentrations of SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL
34 hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL)
35 were not significantly different (P ¡Ý 0.05), but the numbers of total ova/embryos (P < 0.05),
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
33 compare two concentrations of SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL
34 hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL)
35 were not significantly different (P ¡Ý 0.05), but the numbers of total ova/embryos (P < 0.05),
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
31 with a single subcutaneous (SC) injection in SRF (n = 6), while superovulatory response of cows
32 treated with a single IM injection in SRF (n = 6) was intermediate. Experiment 2 was designed to
33 compare two concentrations of SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL
34 hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL)
35 were not significantly different (P ¡Ý 0.05), but the numbers of total ova/embryos (P < 0.05),
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
33 compare two concentrations of SRF (20 mg/mL hyaluronan, 100% compared to 10 mg/mL
34 hyaluronan, 50%) in a single IM injection protocol. The mean number of corpora lutea (CL)
35 were not significantly different (P ¡Ý 0.05), but the numbers of total ova/embryos (P < 0.05),
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
36 fertilized ova (P < 0.01) and transferable embryos (P < 0.001) were greater in cows treated with
37 FSH in 100% SRF (n = 20) than cows treated with FSH in 50% SRF (n = 20). Experiment 3 was
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
39 injection of Folltropin-V diluted in 100% solution of SRF with those treated with the traditional
40 twice-daily IM injection protocol over 4 d. Mean (¡À SEM) numbers of CL (13.7 ¡À 1.2 compared
41 to 13.8 ¡À 1.2), total ova/embryos (12.3 ¡À 1.5 compared to 13.7 ¡À 2.1), fertilized ova (7.2 ¡À 1.1
42 compared to 8.4 ¡À 1.4) and transferable embryos (4.9 ¡À 0.8 compared to 6.4 ¡À 1.3) were not
43 significantly different between Control (n = 29) and Single injection (n = 29) groups,
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
44 respectively. In summary, superstimulation of beef donor cows with a single IM injection of
38 designed to compare superovulatory response in Red Angus donor cows treated with a single IM
