The enzymatic and chemical reduction of extended biliverdins.

FRYDMAN, ROSALÍA BRYKS DE; BARI, SARA E.; TOMARO, MARÍA L.; FRYDMAN, JAIME BENJAMÍN

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 1990 vol. 171 p. 465 - 473

0006-291X

The substrate specificity of rat liver biliverdin reductase was probed usinghelical and extended biliverdins. The former were the ZZZ-all-syn biliverdins IXalpha and IX gamma, and the latter were the 5Z-syn, 10Z-syn, 15Z-anti; 5Z-anti,10E-anti, 15E-anti biliverdins. It was found that the reduction rates of thebiliverdins increased with the progressive stretching of their conformations.The most extended biliverdin was reduced at a higher rate than biliverdin IXalpha. The chemical reduction rates to bilirubins followed a similar pattern.Nucleophilic addition of 2-mercaptoethanol to the C10 methine was also favoredin the extended biliverdins.