Iron Intake and Ancestry in Adults of Buenos Aires

FLEISCHMAN S; PATINO J; JUÁREZ L; DONADÍO L; VELLICCE A; LARDO M; DEJEAN C; AVENA S; LANGINI S

Buenos Aires

Congreso; Reunión De Sociedades de Biociencias; 2020

LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

High iron (Fe) intake in carriers of HFE gene mutations (C282Y, H63D, S65C) can result in overload and eventually, in hereditary hemochromatosis (HH). HH is more common in men than in women. C282Y is characteristic of northern Europe, while H63D is in the Mediterranean countries. European ancestry ís estimated to be 81% in Buenos Aires city. Therefore, given high meat intake in Argentina, ancestry and its relatíonship with possible alterations in Fe metab-olism were studied in clinically healthy adult males. 54 male blood donors (20-58 years) attending Hospital de Clínicas (UBA) (2018) were enrolled. The daily Fe intake (Fel), heme Fe intake (hem Fel) and non-heme Fel intake (non-heme Fel) were evaluated using a food consumption frequency questionnaire (ARGENFOODS and USDA). A genealogical survey was carried out, and ancestry infor-mative markers (AIMs) were studied in whole blood using the mul-tiplex PCR-APLP technique (30 markers). C282Y, H63D and S65C were investigated in blood by DNA extraction (Accupred Genomics DNA Kits) and PCR-RFLP. The Fel, non-heme Fel and heme Fel were, respectively (mg/d): mean ± SD (range): 22.7 ± 8.6 (9.6-47.2); 20.7 ± 7.9 (8.3-42.2); 2.0 ± 1.1 (0.3-5.0). Fortified flours (30 mg Fe/ kg) provided 35.7% of total Fel. The Fel was> 6 mg Fe/d (Estimated Mean Requirement) in all participants, and 3.0% exceeded the Máx-imum Tolerable Intake Level (45 mg Fe/d) (ÑAS, 2001). H63D was present in 13/54 (24%) male donors; only 7/13 (53.8%) answered to have a high European self-perceived ancestry. An average Euro¬pean contribution of 83% (range: 99% - 66%) was estimated by the AIMs. These results suggest that a significant number of clinically healthy individuáis could believe that their European ancestry is few-er than that estimated by a genetic analysis. Therefore, they could be at risk of Fe accumulation given feeding habits in Argentina (55.5 Kg meat /per cápita/y, FAO 2013, and Ley 25630/2002). Universidad de Buenos Aires, UBACyT 20720150100004BA.