First report of grapevine Pinot gris virus infecting grapevine in Argentina

DEBAT, HUMBERTO; LUNA, FACUNDO; MOYANO, SABRINA; ZAVALLO, DIEGO; ASURMENDI, SEBASTIAN; GOMEZ-TALQUENCA, SEBASTIAN

JOURNAL OF PLANT PATHOLOGY

EDIZIONI ETS

Año: 2020 vol. 102

1125-4653

Fourteen viruses have been associated to grapevine inArgentina (Debat et al. 2020; Luna et al. 2019).Grapevine Pinot gris virus (family Betaflexiviridae; genusTrichovirus, GPGV) was originally described inItaly and linked to grapevine leaf mottling and deformation (GLMD) in Vitis vinifera cv. Pinot gr is(Giampetruzzi et al. 2012). In Latin America, GPGVhas been detected in Brazil, Chile and Uruguay.A pooled sample consisting of V. vinifera cv.Torrontés and four criollas varieties from Argentinawere subjected to high-throughput sequencing. TotalRNAs from cambial scrapings were purified using theSpectrum Plant RNA Miniprep Kit (Sigma-Aldrich,USA) and sequenced in an Illumina Novaseq-6000 instrumentgenerating 171,490,780 paired reads. Thesereads were trimmed, filtered and mapped against a collectionof plant virus sequences. 41 150 nt readsmapped to GPGV, covering 8% of its genome and suggestingthe presence of GPGV in our pooled sample.Blastn of the 590 nt-long partial sequence returned100% identity to GPVG isolate Regent-BE fromBelgium (MN228488).To confirm the presence of GPVG, the extracted RNAof each sample was random-primed, retrotranscribed usingM-MLV-RT and subjected to PCR using primersGPgV5619f/GPgV6668r (Giampetruzzi et al. 2012). Onlyone of the tested samples (from V. vinifera cv. TorrontésRiojano) generated an amplification product which wascloned and bi-directionally Sanger-sequenced. The sequenceobtained (MT450471, GPGV-ARG) presented a98.6% highest identity with GPGV isolate BC-1 fromCanada (KU194413), and 98.1% identity with the aforementionedGPGV isolate Regent-BE.The Argentinean isolate of GPGV was found in aplant with no apparent symptoms of viral diseases.This report is of significant importance for theArgentinean viticulture as Colomerus vitis, a widely dispersederiophyd mite and vector of GPGV is present inArgentinean vineyards. To our knowledge, this is thefirst report of GPGV in Argentina.