INVESTIGADORES
ASURMENDI Sebastian
artículos
Título:
Virus infection alters transcriptional activity of miR164a promoter in plants.
Autor/es:
A.A BAZZINI; NI ALMASIA; CA MANACORDA; VC MONGELLI; AJ DISTÉFANO; GA MARONICHE; MC RODRIGUEZ; G CONTI; HE HOPP; M DEL VAS; S ASURMENDI
Revista:
BMC PLANT BIOLOGY
Editorial:
BIOMED CENTRAL LTD
Referencias:
Año: 2009 p. 1 - 12
ISSN:
1471-2229
Resumen:
Abstract
Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have
crucial roles in many important plant functions. Virus infection and transgenic expression of viral
proteins alter accumulation and activity of miRs and so far, most of the published evidence involves
post-transcriptional regulations.Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have
crucial roles in many important plant functions. Virus infection and transgenic expression of viral
proteins alter accumulation and activity of miRs and so far, most of the published evidence involves
post-transcriptional regulations.
Results: Using transgenic plants expressing a reporter gene under the promoter region of a
characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues
and during Arabidopsis development. Strong expression was detected in both vascular tissues and
hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum
expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to
reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic
virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we
demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total
mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after
virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter
induction.Using transgenic plants expressing a reporter gene under the promoter region of a
characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues
and during Arabidopsis development. Strong expression was detected in both vascular tissues and
hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum
expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to
reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic
virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we
demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total
mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after
virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter
induction.Arabidopsis development. Strong expression was detected in both vascular tissues and
hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum
expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to
reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic
virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we
demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total
mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after
virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter
induction.Tobacco mosaic
virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we
demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total
mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after
virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter
induction.Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we
demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total
mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after
virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter
induction.
Conclusion: This work shows for the first time that the alteration of miR pathways produced by
viral infections possesses a transcriptional component. In addition, the degree of miR alteration
correlates with virus severity since a more severe virus produces a stronger P-miR164a induction.This work shows for the first time that the alteration of miR pathways produced by
viral infections possesses a transcriptional component. In addition, the degree of miR alteration
correlates with virus severity since a more severe virus produces a stronger P-miR164a induction.