Differential mRNA accumulation upon early Arabidopsis thaliana infection with ORMV

ZAVALLO, D; DEBAT, H; CONTI, G; MANACORDA CA; RODRIGUEZ MC; ASURMENDI S

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2015

1932-6203

Small RNAs (sRNAs) play important roles in plant development and host-pathogeninteractions. Several studies have highlighted the relationship between viral infections,endogenous sRNA accumulation and transcriptional changes associated withsymptoms. However, few studies have described a global analysis of endogenoussRNAs by comparing related viruses at early stages of infection, especially before viralaccumulation reaches systemic tissues.An sRNA high-throughput sequencing of Arabidopsis thaliana leaf samples infectedeither with Oilseed rape mosaic virus (ORMV) or crucifer-infecting Tobacco mosaicvirus (TMV-Cg) with slightly different symptomatology at two early stages of infection (2and 4dpi) was performed. At early stages, both viral infections strongly alter thepatterns of several types of endogenous sRNA species in distal tissues with no virusaccumulation suggesting a systemic signaling process foregoing to virus spread. Acorrelation between sRNAs derived from protein coding genes and the associatedmRNA transcripts was also detected, indicating that an unknown recursive mechanismis involved in a regulatory circuit encompassing this sRNA/mRNA equilibrium. Thiswork represents the initial step in uncovering how differential accumulation ofendogenous sRNAs contributes to explain the massive alteration of the transcriptomeassociated with plant-virus interaction