INVESTIGADORES
ASURMENDI Sebastian
artículos
Título:
A practical approach to the understanding and teaching of RNA silencing
Autor/es:
BAZZINI, A. A.; MONGELLI, V. C.; HOPP, E.; DEL VAS, M.; SEBASTIAN ASURMENDI
Revista:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Referencias:
Año: 2007 vol. 10 p. 178 - 190
ISSN:
0717-3458
Resumen:
Gene silencing, also called RNA interference (RNAi) is a
specific mechanism of RNA degradation involved in
gene regulation, development and defense in eukaryotic
organisms. It became an important subject in the
teaching programs of molecular biology, genetics and
biotechnology courses in the last years. The aim of this
work is to provide simple and inexpensive assays to
understand and teach gene silencing using plants as
model systems. The use of transient and permanent
transgenic plants for expressing reporter genes, like
those derived from jellyfish green fluorescent protein
(gfp) encoding gene, provides a nice, colorful and
conclusive image of gene silencing. Three experimental
approaches to evidence RNA silencing are depicted. In
the first approach gene silencing is demonstrated after
transient expression of reporter genes in non-transgenic
plants. In the second, silencing is triggered against a
reporter gene stably integrated into a transgenic plant.
The third approach involves the triggering of RNA
silencing against endogenous genes using viral vectors.
In addition we illustrate systemic gene silencing showing
how the silencing signal is spread over a plant and
finally it is also demonstrated the suppression of gene
silencing. The first group of experiments is
recommended to be tough on undergraduate courses,
the following two sections are recommended for
graduate courses. Hopefully, it will help students to
understand this important phenomenon and to unravel
the importance of gene silencing as a key gene
regulation mechanism and as a molecular and
biotechnological tool.
conclusive image of gene silencing. Three experimental
approaches to evidence RNA silencing are depicted. In
the first approach gene silencing is demonstrated after
transient expression of reporter genes in non-transgenic
plants. In the second, silencing is triggered against a
reporter gene stably integrated into a transgenic plant.
The third approach involves the triggering of RNA
silencing against endogenous genes using viral vectors.
In addition we illustrate systemic gene silencing showing
how the silencing signal is spread over a plant and
finally it is also demonstrated the suppression of gene
silencing. The first group of experiments is
recommended to be tough on undergraduate courses,
the following two sections are recommended for
graduate courses. Hopefully, it will help students to
understand this important phenomenon and to unravel
the importance of gene silencing as a key gene
regulation mechanism and as a molecular and
biotechnological tool.
conclusive image of gene silencing. Three experimental
approaches to evidence RNA silencing are depicted. In
the first approach gene silencing is demonstrated after
transient expression of reporter genes in non-transgenic
plants. In the second, silencing is triggered against a
reporter gene stably integrated into a transgenic plant.
The third approach involves the triggering of RNA
silencing against endogenous genes using viral vectors.
In addition we illustrate systemic gene silencing showing
how the silencing signal is spread over a plant and
finally it is also demonstrated the suppression of gene
silencing. The first group of experiments is
recommended to be tough on undergraduate courses,
the following two sections are recommended for
graduate courses. Hopefully, it will help students to
understand this important phenomenon and to unravel
the importance of gene silencing as a key gene
regulation mechanism and as a molecular and
biotechnological tool.
gfp) encoding gene, provides a nice, colorful and
conclusive image of gene silencing. Three experimental
approaches to evidence RNA silencing are depicted. In
the first approach gene silencing is demonstrated after
transient expression of reporter genes in non-transgenic
plants. In the second, silencing is triggered against a
reporter gene stably integrated into a transgenic plant.
The third approach involves the triggering of RNA
silencing against endogenous genes using viral vectors.
In addition we illustrate systemic gene silencing showing
how the silencing signal is spread over a plant and
finally it is also demonstrated the suppression of gene
silencing. The first group of experiments is
recommended to be tough on undergraduate courses,
the following two sections are recommended for
graduate courses. Hopefully, it will help students to
understand this important phenomenon and to unravel
the importance of gene silencing as a key gene
regulation mechanism and as a molecular and
biotechnological tool.