INVESTIGADORES
ARREGUI Carlos Oscar
artículos
Título:
PTP1B regulates neurite extension mediated by cell-cell and cell-matrix adhesion molecules
Autor/es:
PATHRE, PURNIMA; ARREGUI, CARLOS; WAMPLER, THERESA; KUE, IA; LEUNG, TINCHUNG; CARLOS OSCAR ARREGUI
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Editorial:
Wiley Liss
Referencias:
Año: 2001 vol. 63 p. 143 - 150
ISSN:
0360-4012
Resumen:
N-cadherin and b1-integrin adhesion and signaling play
important roles in growth cone adhesion and guidance.
Each of these adhesion receptor systems is composed of
multiprotein complexes, and both adhesion and downstream
signaling events are regulated through the interaction
of protein tyrosine kinases and phosphatases with
many of the proteins that make up these complex systems.
Work from our laboratory reported that the nonreceptor
protein tyrosine phosphatase PTP1B is localized
to adherens junctions and focal adhesion complexes and
regulates both N-cadherin- and b1-integrin-mediated
adhesion. PTP1B appears to modulate integrin-mediated
adhesion through regulation of src activation and
cadherin-mediated adhesion through dephosphorylation
of b-catenin. We have continued these studies and report
that PTP1B is localized to the tips of growing neurites
and that introduction of a noncatalytic mutant of
PTP1B into PC12 cells results in inhibition of N-cadherin and
b1-integrin-mediated neurite outgrowth but is without
effect on neurite outgrowth on poly-L-lysine. Moreover,
suppressing the level of PTP1B in primary embryonic
chick neural retina cells using antisense
oligonucleotides also inhibits N-cadherin- and b1-
integrin-mediated neurite outgrowth. Neither of these
techniques reduces the levels of expression of either
adhesion receptor. We conclude that PTP1B is a regulatory
component of the molecular complex required for
both N-cadherin and b1-integrin-mediated axon growth.