Long-lasting protective immune response elicited by vaccination with an expression genomic library of Toxoplasma gondii

FACHADO, A.; RODRIGUEZ, A.; MOLINA, J.; SILVERIO, J.; MARINO, A.; PINTO, L.; SERGIO OSCAR ANGEL; INFANTE, J.; TRAUB-CSEKO, Y.

INFECTION AND IMMUNITY

Año: 2003 vol. 71 p. 5407 - 5411

0019-9567

BALB/c mice were intramuscularly immunized with low doses (25-50microg) of DNA cocktail containing plasmids encoding the full-length SAG1/P30 and the 196-561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-gamma) secretion, whereas no IL-4 was detected. Moreover, DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T. gondii DNA vaccine.