INVESTIGADORES
ANDREU Adriana Balbina
congresos y reuniones científicas
Título:
. Interaction between serin and aspartic proteases involved in plant defense.
Autor/es:
SUELDO D; ANDREU A. B; DALEO G; GUEVARA M G.; OLIVIERI F. P.
Lugar:
Mar del Plata. Argentina
Reunión:
Congreso; SAIB 43th Annual Meeting; 2007
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
In previous reports it has been showed that FESP, a Fusarium
eumartii extracellular serin protease, is able to degrade, in vitro,
a potato aspartic protease, StAP1, detected in intercellular washing
fluids (IWF) after 1 day of wounding (1dW). Such interaction was analyzed in
Pampeana, a cultivar susceptible to F. eumartii infection. In order to
know the role of this interaction in the potato-F. eumartii pathosystem,
the accumulation of both proteases was analyzed in Spunta, a moderately
resistant cultivar. Results indicated that the pattern of accumulation of FESP
and StAP1 in Spunta was similar to the one previously obtained in
Pampeana, though the timing of expression differed, being StAP1 more
persistent in Spunta than in Pampeana. On the other hand, in an attempt to
determine if this proteolytic interaction affects StAP1 antimicrobial
activity, F. eumartii conidia were incubated in the presence of StAP1
or StAP1 plus FESP and germination was evaluated. As expected, conidia
failed to germinate when incubated with 3.75 uM StAP1, although they did
in the presence of both proteases or FESP alone. This result show that the
proteolytic interaction between StAP1 and FESP affects StAP1
inhibitory effect on F. eumartii conidia, suggesting a possible in
vivo role, in which the StAP1 antimicrobial activity could be
regulated by the accumulation of FESP in infected tissue.
Financiado
por: CONICET, UNMDP