ANTIOXIDANT PROTECTIVE EFFECT OF POTATO PEEL POLYPHENOLS.

FILIBERTI V; VAZQUEZ M; ANDREU A. B.; SILVEYRA M.X.

Mar del Plata

Congreso; Reunión de Biociencias SAIC, SAI, FAIC & SAFIS 2022; 2022

SAIC, SAI, FAIC y SAFIS

Oxidative stress is associated with many pathologies, such as neurodegenerative diseases, and polyphenols are antioxidants that could prevent this stress. Potato peel waste (PPW) is an abundant leftover from the potato processing industry. We have previously identified polyphenols in extracts from PPW, such as chlorogenic acid, caffeic acid, and ferulic acid. This study aimed to analyze the antioxidant properties of PPW in vitro and evaluate its neuroprotective effect on cell-injured by oxidative stress. First, we compared different solvents to optimize the polyphenolic extraction (ethanol-water 80%, 50%, and water), analyzing the total phenolic content and the antioxidant capacity. We also identified some of the compounds by the DAD HPLC method. We observed that the compounds and their proportion were different according to the ethanol content of the extracts. However, chlorogenic acid represented 95% of the composition in all of them. To test the neuroprotective activity, we assayed different concentrations of PPW extract in vitro oxidative stress model. First, we observed that low concentrations of PPW extracts were not cytotoxic and slightly increased the mitochondrial membrane potential ∆ѱmit). We also demonstrated that pretreatment with these concentrations of PPW extracts protected injured oxidative stress cells. Our results showed that PPW polyphenols restored the ∆ѱmit and ROS levels modified by the injured-oxidative stress and protected cells from cell death. These findings suggest that potato peel waste would be a good source of neuroprotective antioxidant polyphenols to develop a dietary supplement with some impact on human health