INVESTIGADORES
ALVAREZ Luis Ignacio
artículos
Título:
Uptake of albendazole and albendazole sulphoxide by Haemonchus contortus and Fasciola hepatica in sheep.
Autor/es:
ALVAREZ, L., IMPERIALE, F., SÁNCHEZ, S., MURNO, G., LANUSSE, C.
Revista:
VETERINARY PARASITOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2000 vol. 94 p. 75 - 89
ISSN:
0304-4017
Resumen:
The pattern o f in vivo
uptake of albendazole (ABZ) and its major metabolite, ABZ-sulphoxide (ABZSO),
by Haemonchus contortus and Fasciola hepatica recovered from
ABZ-treated sheep was investigated. Concentration profiles of both compounds
were simultaneously measured in target tissues/fluids from the same infected
sheep. In addition, the proportion of the (+) and (-) ABZSO enantiomers was
determined in plasma, bile and F. hepatica recovered from treated sheep.
H. contortus naturally infected sheep were intraruminally (i.r.) treated
with ABZ (micronized suspension, 7.5 mg/kg) and the plasma concentrations of
ABZSO and ABZ-sulphone (ABZSO2) determined in addition to the
concentration of ABZ and ABZSO in H. contortus, abomasal mucosa and
fluid content samples. In addition, F. hepatica artificially infected
sheep were treated i.r. with the same ABZ suspension (7.5 mg/kg), and samples
of blood, bile, liver tissue and adult flukes were collected and analysed by
HPLC to determine the concentrations of ABZ and both enantiomers of ABZSO. ABZSO
and ABZSO2 were the analytes recovered in plasma with ABZ and ABZSO
present in H. contortus. ABZ was the analyte recovered at the highest
concentration in H. contortus and abomasal mucosa, whereas higher
concentrations of ABZSO were measured in abomasal fluid content. Only low
concentrations of ABZ were detected in F. hepatica and bile, but
markedly higher concentrations of ABZ were measured in liver tissue. ABZSO was
the main molecule recovered in F. hepatica, plasma and bile samples
collected from ABZ-treated sheep. The (+) enantiomer of ABZSO was recovered at
a higher proportion in plasma (75 %), bile (78 %) and F. hepatica (74 %)
after ABZ administration to infected sheep.