ALVAREZ Luis Ignacio
Chemoprophylactic activity of flubendazole in cystc echinococcosis
CEBALLOS, L.; ELISSONDO, M.; SÁNCHEZ BRUNI, S.; CONFALONIERI, A.; DENEGRI, G.; ALVAREZ, L.; LANUSSE, C.
Cystic echinococcosis (CE) is an important public health problem worldwide. Flubendazole, administered as a suspension, has shown poor in vivo efficacy against CE in humans. However, flubendazole prepared as a solution caused a marked reduction in hydatid cysts developed in mice. The goal of the current work was to compare the chemoprophylactic effect of flubendazole formulated either as a hydroxipropyl-β-cyclodextrin solution or as a carboxymethylcelullose suspension in secondary CE in mice. Methods: Balb/c mice were infected with E. granulosus protoscoleces. One day after infection the animals were allocated into three (3) different experimental groups: unmedicated control and treated at the time point of infection with flubendazole either prepared as a solution or suspension given twice a day during 15 days. Six months (6) post-infection, the animals were sacrificed to collect and weight parasite cysts. Cyst samples recovered from infected mice of each experimental group were prepared for both scanning and transmission electron microscopy. Results: Both flubendazole formulations induced a significantly reduction in cysts weight compared to those recovered from the unmedicated control animals. Both formulations showed similar flubendazole-induced ultrastructural morphological changes. Conclusion: Flubendazole offers a great potential to become a drug of choice in the preventive treatment of cystc echinococcosis.