CONICET | Buscador de Institutos y Recursos Humanos

One of the main causes of greenhouse effect is the industrial emissions of CO2. Moving away from fossil energy sources to more sustainable ones is important to alleviate this issue. The use of one-carbon (C1) compounds as feedstock for biomanufacturing is a promising new alternative. For this reason, it is important to study the C1 fixation and assimilation pathways as well as the biological adaptations of microorganisms under oligotrophic conditions.In this study we analyzed the response of Rhodococcus aetherivorans, an actinobacteria with a wide biotechnological potential, to grow in oligotrophic conditions using 5% of a waste gas mixture composed of 96% CO2 and 2% CH4 as sole carbon source (WGM). We performed a transcriptomic analysis (RNAseq) focusing on different metabolic and regulatory pathways associated with oligotrophic growth in comparison to heterotrophic growth (glucose 1%, p/v). Both samples were cultivated on mineral salt medium with 0.1 g/l ammonium (MSM0.1) and collected during exponential phase of growth.Transcriptomic results revealed that 48.36% of the total genes significantly modified their expression profiles under oligotrophy. This condition led to a significant turnover of regulatory components, such as the up- and down-regulation of several transcriptional regulators, two component systems and Sigma factors, indicating that the metabolic modifications in cells under oligotrophy are highly regulated by a still unknown regulatory circuit. A dramatic metabolic reorganization has been observed in cells represented by: (1) down-regulation of several genes of the sugar-P, central metabolism, TCA cycle and turnover of components of the respiratory chain, with a significant reduction of the metabolic activity (

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