INVESTIGADORES
ALVAREZ Hector Manuel
artículos
Título:
Lipid storage compounds in marine bacteria
Autor/es:
ALVAREZ, H. M.; HÉCTOR PUCCI, OSCAR; STEINBÜCHEL, A
Revista:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Editorial:
Springer
Referencias:
Lugar: Berlin; Año: 1997 vol. 47 p. 132 - 139
ISSN:
0175-7598
Resumen:
Abstract Forty psychrophile or psychrotrophic crudeoil-
utilizing marine bacteria were investigated for their
ability to accumulate lipid storage compounds in the
cytoplasm during cultivation under nitrogen-limiting
conditions. Most of them (73%) were able to accumulate
specialized lipids like polyhydroxyalkanoic acids (PHA)
while other lipids such as wax esters occurred in two
isolates. Accumulation of PHA occurred predominantly
at low temperatures (4±20 °C) as demonstrated for three
isolates. Electron microscopy revealed polyphosphate
inclusions occurring in two isolates in addition to PHA.
Cells of the isolate Acinetobacter sp. 211 were able to
synthesize and accumulate lipid inclusions during
growth on acetate, ethanol, olive oil, hexadecanol and
heptadecane. The composition of the lipid inclusions
depended on the compounds provided as carbon source.
Wax esters and acylglycerols occurred mainly during the
cultivation on olive oil; in contrast, wax esters and free
alcohols occurred during cultivation on hexadecanol.
Total fatty acids in cells of the Acinetobacter sp. 211
amounted to 25% of the cellular dry weight in olive-oilgrown
cells. Palmitic acid was the main fatty acid in the
lipids when the cells were cultivated on acetate or
ethanol (44% and 32% of total fatty acids respectively).
In contrast, fatty acids occurring in the lipids during
cultivation on hexadecanol, heptadecane or olive oil
were related to the carbon source. The fatty acids present
in the accumulated lipids consisted predominantly
of saturated and unsaturated straight-chain fatty acids
with a chain length ranging from 12 to 18 carbon atoms.
Analysis of the lipid-granule-associated proteins in cells
of Acinetobacter sp. 211 revealed a protein of 39 kDa as
the predominant protein species.