POSTER en WORKSHOP

ROZÉS-SALVADOR, VICTORIA; ALVAREZ, CECILIA

Santa Cruz

Workshop; EMBO Workshop Emerging Concepts of the Neuronal Cytoskeleton; 2023

IUBMB EMBO

CREB3L1 belongs to the CREB3 family of transcription factors that are implicated in the ER and Golgistress responses as regulators of the cell secretory capacity and cell specific cargos. In response todifferent signals, CREB3 proteins are transported from the ER to the Golgi complex where they arecleaved (activated) by S1P and S2P proteases sequentially. Although CREB3 factors have a widerange of biological functions, their role in neuronal development is not well understood. In our study, weobserved that CREB3L1 localizes to the basal bodies of primary cilia during early neuronal celldevelopment. Primary cilia are sensory organelles that project from the plasma membrane of many celltypes, including neurons. They play an important role in intracellular signaling pathways and act assensory organelle for extracellular and intracellular signals. Our preliminary results show that CREB3L1colocalizes with γ-tubulin and inversin, a protein located in the basal body of primary cilia at earlyculture times (3DIV). This basal body localization is lost when CREB3L1 activation by S1P and S2P proteases is inhibited. Our findings suggest that CREB3L1 may have a non-canonical function inprimary cilia that is important for neuronal development and function. Further research on this topiccould provide new insights into the mechanisms underlying neuronal function.Funding:PICT 2019-1625 (CA), PICT 2020-00995 (VRS)