One step derivatization and preconcentration microextraction technique for determination of bisphenol A in beverage samples by gas chromatography–mass spectrometry

ARIEL R. FONTANA; MÓNICA MUÑOZ DE TORO; JORGELINA C. ALTAMIRANO

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Año: 2011 p. 3559 - 3565

0021-8561

A rapid and efficient technique based on ultrasound-assisted emulsification microextraction (USAEME) followed by solvent-assisted emulsion disruption (SAED) without centrifugation is proposed for extraction and preconcentration of bisphenol A (BPA) in environmental water samples. BPA was in-situ derivatized with acetic anhydride and determined by gas chromatography-mass spectrometry (GC–MS). After emulsification, two clear phases were obtained without centrifugation by disrupting the emulsion adding acetonitrile as emulsion disrupter. Variables influencing the extraction efficiency of BPA were studied and optimized. Under optimal experimental conditions, the detection limit (LOD) was 1.2 ng L-1 with a relative standard deviation (RSD) value of 6.6 % (n = 5) when 5 ng L-1 BPA standard mixture was analyzed. The linear working range was 5-1500 ng L-1 and the coefficient of estimation of the calibration curve obtained following the proposed methodology was ≥0.9992. The robustness of the proposed methodology was assayed by carrying out a recovery study at three concentrations (5, 50 and 500 ng L-1). This study led to a satisfactory result achieving recoveries ≥92 % which showed a successfully forcefulness for determination of nanogram per liter of BPA in water samples of environmental interest.