Chemoattractant Effect of Ethanolic Extract of Lentinus tigrinus on Respiratory Tract in Mice.

ALBERTÓ E.; BAGDADI, A; FERNANDEZ ALANIS E; MARTIN S; MANDALUNIS P; TASAT D.

Congreso; 8th Medicinal Mushrooms Conference; 2015

The increase of immune disorders ofthe respiratory tract related with environmental pollution makes necessary thedevelopment of preventive actions to reduce the severity of these diseases. Extracts of mushrooms have long been said to be efficacious in treating variousdiseases since they are rich in bioactive compounds that act asimmunopotentiators, immunoiniciators and biological response modifiers. Lentinus tigrinus (LT) is a xilophagousmushroom that grows from the centre to the north of Argentina. This species canbe easily cultivated on sawdust with high yields. In this work, we evaluated in vivo the preventive potential effectof an ethanolic extract (LTF1) obtained from LT, on the immflamatory responseinduced by ROFA (residual oil fly ash) on Balb/C mice. Animals received 6 intranasal dosesof LTF1 (0.085, 0.170 and 0.340 mg/Kg) for two weeks followed by 3 daily nosesof ROFA (0.170 mg/Kg) three times for one week. Pulmonary immflamatory responsewas assessed by a) the histological observation of the number of goblet cells(GC) and b) total and differencial (macrophages and polumorphonuclear cellcount (TCC and PMN respectively) in bronchioalveolar lavage (BAL).   The bronchoalveolar lavage inBalb/C mice treated with LTF1 showed a significative increase of the TCRcompared to control. The percentage of macrophages (MA), PMN and oxygenreactive species (ROS) remained the same. Furthermore, we did not observebronchial epithelial stratification or changes in the number of goblet cells inthe histological studies. In the bronchoalveolar lavage inBalb/C mice treated with ROFA we observed a significative increase in TCC, thepercentage of MA and the generation of ROS. Moreover, we noticed bronchialepithelial stratification and an increase in the number of goblet cells, in thehistological studies.The animals receiving combinedtreatment (LTF1 + ROFA) showed an increase of TCC and percentage of PMN. On theother side, the generation of ROS decreaced when compared with ROFA.  In the histological studies, we did not findbronchial epithelial stratification; the number of goblet cells showed a slightdecrease when compared with animals treated with ROFA.The RCT increases and variations ininflammatory parameters studied could suggest a potent chemoattractant activitybut a mild anti-inflammatory activity of LLF1.