Effects of LRE1, an inhibitor of soluble adenylyl cyclase, on ischemia-reperfusion injury in isolated rat heart.

CIOCCI PARDO A; MARIÁNGELO JIE; AIELLO EA; MOSCA SM

Congreso; Reunión Anual SAFIS-ALACF 2021 (virtual); 2021

Introduction: The role of soluble adenylyl cyclase (sAC, one of the sources of cAMP) in cell signaling has been widely studied, its participation in ischemia-reperfusion is not clear. Objective: To examine the effects of LRE1, a sAC inhibitor, on myocardial postischemic alterations. Methods: Isolated perfused rat hearts were assigned, after 20 min of stabilization, to the following groups: 1) Non-ischemic control (NIC): 90 min of perfusion; 2) Ischemic control (IC): 30 min of global ischemia (GI) and 60 min of reperfusion (R), 3) LRE1: 10 μM LRE1 was administered during the initial 10 min of R. Infarct size (IS) was measured by TTC staining. Left ventricular developed pressure (LVDP) and left ventricle end-diastolic pressure (LVEDP) were used to assess systolic and diastolic function, respectively. Other hearts (n =3) were submitted to 30 min of GI and 10 min of R, in absence and in presence of LRE1. The lactate dehydrogenase (LDH) release during 10 min of R was measured. In isolated mitochondria, mitochondrial state was assessed through the Ca2+ retention capacity (CRC, Calcium Green 5N). Data were given as means ± SEM and analyzed using ANOVA followed by Turkey´s test. A p