The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury.

FANTINELLI JC; ORLOWSKI A; AIELLO EA; MOSCA MS

CARDIOVASCULAR PATHOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2014 vol. 23 p. 224 - 230

1054-8807

Background Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia?reperfusion is still unclear. Methods and results Our objective was to assess the role of NBCe1 in reperfusion injury. We use selective functional antibodies against extracellular loop 3 (a-L3) and loop 4 (a-L4) of NBCe1. a-L3 inhibits and a-L4 stimulates NBCe1 activity. Isolated rat hearts were submitted to 40 min of coronary occlusion and 1 h of reperfusion. a-L3, a-L4 or S0859 ? selective Na+-HCO3- co-transport inhibitor ? were administered during the initial 10 min of reperfusion. The infarct size (IS) was measured by triphenyltetrazolium chloride staining technique. Postischemic systolic and diastolic functions were also assessed. a-L3 and S0859 treatments decreased significantly (P